Novel sensitive real-time PCR for quantification of bacterial 16S rRNA genes in plasma of HIV-infected patients as a marker for microbial translocation

M Kramski; AJ Gaeguta; GF Lichtfuss; R Rajasuriar; SM Crowe; MA French; SR Lewin; RJ Center; DFJ Purcell

Journal article

Novel sensitive real-time PCR for quantification of bacterial 16S rRNA genes in plasma of HIV-infected patients as a marker for microbial translocation

M Kramski, AJ Gaeguta, GF Lichtfuss, R Rajasuriar, SM Crowe, MA French, SR Lewin, RJ Center, DFJ Purcell

Journal of Clinical Microbiology | Published : 2011

DOI: 10.1128/JCM.01018-11

Abstract

We developed a real-time PCR to quantify 16S rRNA gene levels in plasma from HIV-infected patients as a marker of microbial translocation. The assay uses shrimp nuclease (SNuc) to eliminate DNA contamination, giving high sensitivity and low variability. The 16S rRNA gene levels measured in plasma from HIV patients correlated significantly with lipopolysaccharide levels. Copyright © 2011, American Society for Microbiology. All Rights Reserved.

University of Melbourne Researchers

Reena Rajasuriar Author

Sharon Lewin Author

Damian Purcell Author

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Grants

Awarded by National Health and Medical Research Council of Australia

Awarded by Career Development Award

Funding Acknowledgements

This work was supported by the National Health and Medical Research Council of Australia (grants 400302 and 510488) and by Career Development Award 359233 to R.J.C. S.R.L. is an NHMRC practitioner fellow and receives funding from the Alfred Foundation.